5. May 2021 FBS Market Survey – Test Welcome to your FBS Market Survey - Test. Your know-how is required! As a company manufacturing human-derived cell culture supplements as alternatives to Fetal Bovine Serum (FBS), we are interested in current developments of your lab work. To gather insights that help us to improve our Human Platelet Lysates (HPL), we ask you to participate in our survey. Your input is highly valued and you take the opportunity to win: Among all participants, we will raffle three Amazon gift cards valued 50 €. This survey will take you approximately 10 minutes to answer the multiple-choice questions. *Required fields Terms and Conditions: You can only once participate in the survey. Only one gift card per person is raffled. The draw ends on 30 June 2021 at 23:59. The participants have to be at legal age. The winners are drawn by random principle and contacted via Email. The judges’ decision in terms of drawing the winners and possible evaluation of the submitted fee is final. Name* Last name* E-mail* 1. What kind of organisation do you work for?Academia/UniversityIndustry (Biotech company, pharmaceutical company)Educational institutionGovernmental institutionOther 2. What country do you live in? 3. What is your age?Between 18 and 25Between 25 and 35Between 35 and 45Between 45 and 55Between 55 and 65Prefer not to answer 4. What are your main professional or research activities?You can choose more than one answer. Basic research Translational/applied research Clinical research Clinical application/cell therapy Production Contract Manufacturing Organisation/Contract Development Manufacturing Organisation (CMO/CDMO) Distribution Other 5. What is your role in the organisation?ProfessorPostdoc ResearcherStudent/PhD StudentResearch AssistantHead of DepartmentSenior Scientist/Team LeaderLaboratory TechnicianManagerSalesBusiness DevelopmentCommunication/MarketingOther 6. How familiar are you with cell culture techniques?Expert levelWell-advancedCompetent levelBeginner levelNo experience 7. Which cells do you expand or are expanded in your organisation?You can choose more than one answer. Human primary cells Human cell lines Animal primary cells Animal cell lines Stem cells Bacteria Other 8. How long have you been working in cell culture?Less than 2 years2 - 5 years5 - 10 yearsLonger than 10 years 9. What is your personal aim when performing cell culture?You can choose more than one answer. Publish my results Develop therapies Build a reputation within my research area Have a positive impact on society Have a positive impact on the environment (sustainability) Support animal welfare Help my organisation moving forward Contribute to the scientific/medical progress 10. Are you currently working with Fetal Bovine Serum (FBS)?YesNoNot currently, but in the past 11. How long have you been working with FBS?Less than 2 years2- 5 years5-10 yearsMore than 10 yearsI have not worked with FBS so far 12. Do you plan to work with FBS in the future?Yes, I will continue to work with FBSYes, I will start to work with FBSNoMaybeOther considerations 13. Are you aware of the fact that FBS extraction is not permitted within the EU?The extraction of FBS for commercial purposes is not allowed within the EU, therefore it is outsourced in countries with lower ethical hurdles and a lower awareness for animal welfare.Yes, of courseNo 14. Of what origin is the FBS you use?With 'origin', we mean the country of serum extraction, not the country of approval or further processing. Please keep in mind that FBS extraction is not allowed in several European countries, so the potential statement "EU origin" only refers to the country of approval or further processing which is not the origin. North America South America Australia New Zealand South Africa I don't know Other 15. From which company do you source FBS? Atlas Biologicals, Inc. Avantor, Inc. BI – Biological Industries Biomol GmbH Bio-Techne Biowest Nucleus Biologics Caisson Laboratories Inc. Cell Culture Technologies LLC Corning HyClone GE Healthcare Gibco HiMedia Laboratories Pvt. Ltd. LSG – Life Science Group Merck KGaA (Sigma Aldrich) Nucleus Biologics PAN-Biotech Rocky Mountain Biologicals Takara Bio Inc. TCB – Tissue Culture Biologicals Thermo Fisher Scientific Inc 16. You did not find the company in the list? Please type in the company here: 17. How is the FBS you use treated?You can choose more than one answer. I use non-treated FBS Heat-inactivated Gamma-irridated Endotoxin-free Qualified for a specific cell type Exosome-depleted Don't know 18. Do you perform batch tests before purchasing FBS?Yes, every timeSometimesNo 19. What price do you pay per 500 mL FBS?Less than 200 $ (160 €)From 200 - 400 $ (160 - 330 €)From 400 - 700 $ (330 - 580 €)From 700 - 1,000 $ (580 - 830 €)More than 1,000 $ (830 €)Don't knowPrefer not to answer 20. Do you pay the list price or a discounted price?List priceDiscountedDon't knowPrefer not to answer 21. How much FBS (litres) do you use per year in your organisation?Less than 10 L10 to 25 L25 - 50 LMore than 50 LDon't know 22. How much FBS (litres) do you usually store per batch?Up to 5 L5 - 10 L10 - 25 LMore than 25 LDon't know 23. How long does the stored FBS volume last?About half a yearAbout a yearAbout two yearsMore than two yearsDon't know 24. Are you aware of the extraction process of FBS?Doctors Against Animal Experiments Germany describes the process as follows: "Immediately after slaughtering a pregnant cow, the fetus is cut out of her uterus. Then a big needle is stabbed between the ribs through the skin and muscles directly into the beating heart of the still living calf. Its blood is aspirated until the animal remains bloodless and dies. This procedure is performed without anesthesia, although it is considered that calf fetuses are already capable of suffering."Source: https://www.aerzte-gegen-tierversuche.de/en/resources/animal-free-research/84-fcs-free-culture-media-without-fetal-calf-serumYes, of courseNo 25. Do you see an ethical issue in the use of FBS?YesNo 26. Do you recognize supply shortage of FBS?YesSomehowNo, not yet 27. Do you recognize a price increasement of FBS?YesSomehowNo, not yet 28. Which challenges do you face when using FBS?You can choose more than one answer. Inconsistency between batches Lack of reproducibility Supply shortage Price increase Limited ability to translate research results into clinical uses 29. Which animal-free FBS alternatives do you know?You can choose more than one answer. Human Serum (HS) Commercial Human Platelet Lysate (HPL) Self-produced Human Platelet Lysate (HPL) Synthetic media (chemically defined media) Others 30. Where do/would you search for information on animal-free supplements?You can choose more than one answer. Online search Journals/papers Event/conference Social media Recommendations/colleagues Other 31. Have you tested or considered using animal-free supplements that have been demonstrated to achieve comparable growth results as FBS?Yes, of courseNo, not yet 32. Which FBS alternatives have you tested?You can choose more than one answer. Human Serum Commercial Human Platelet Lysate (HPL) Self-produced Human Platelet Lysate (HPL) Chemically defined media Others None 33. When selecting a cell culture supplement, which parameter is MOST important to you?You are welcome to state other important parameters in the comment field.Supply continuityLot-to-lot consistencyPrice stabilityCell culture performanceReproducible resultsTranslation of research results into clinical usesProduct integrity 34. Would you be willing to pay more for an animal-free FBS alternative?If the alternative... ...is proven to achieve comparable or better cell growth results than FBS ...is proven to be more sustainable and environmental-friendly than FBS ...has no ethical concerns related to the raw material/production YesDepends on how much moreNo 35. Are you interested to receive material on the production process and difference of HPL and FBS? (Publications and papers.)Yes, please send it to my e-mail addressNo, thank you 36. Would you like to test our Human Platelet Lysate (ELAREM™ Prime) as an animal-free alternative to FBS?Yes, I would like to get a free sampleNo thank you 37. Consent to data processing and usage* I hereby agree that the personal information I have provided voluntarily (name and e-mail) may be raised, processed and used in order to provide the information from this survey. I consent to be contacted by PL BioScience GmbH by e-mail if needed, e.g. to get informed about the draw results. 38. Do you have a message or question for us? Just type it in and we will come back to you.You can withdraw your consent by writing an e-mail to firstname.lastname@example.org at any time. You find further information in our data privacy declaration. 39. Do you want to get subscribed to our newsletter?Don't worry, we will not harass you with loads of mails. Instead, we will provide you with scientific news about HPL, our products and sales activities. Yes, pleaseNo, thank you Please fill in the comment box below. Time is Up!