Publications

Microphysiological System-Generated Physiological Shear Forces Reduce TNF-α-Mediated Cartilage Damage in a 3D Model of Arthritis.
Damerau A., Nguyen D.H.D., Lubahn C., et al. (2025). Adv Sci (Weinh). 2025 Feb;12(7):e2412010.
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2024

GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy.
Terheyden-Keighley D., Hühne M., Berger T., et al. (2024). Stem Cells Transl Med. 2024 Sep 10;13(9):898-911.
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Differential Smad2/3 linker phosphorylation is a crosstalk mechanism of Rho/ROCK and canonical TGF-β3 signaling in tenogenic differentiation.
Melzer M., Niebert S., Heimann M., et al. (2024). Sci Rep. 2024 May 6;14(1):10393.
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Development of a novel high-throughput culture system for hypoxic 3D hydrogel cell culture.
Egger D., Baier L., Moldaschl J., et al. (2024). Sci Rep. 2024 Apr 30;14(1):9904.
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The fluorochrome-to-protein ratio is crucial for the flow cytometric detection of tissue factor on extracellular vesicles.
Weiss R., Mostageer M., Eichhorn T., et al. (2024). Sci Rep. 2024 Mar 17;14(1):6419.
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A Combination of Flexible Modified Plant Virus Nanoparticles Enables Additive Effects Resulting in Improved Osteogenesis.
Schuphan J., Stojanović N., Lin Y.Y., et al. (2024). Adv Healthc Mater. 2024 Feb 28:e2304243.
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The Phenotype of Mesenchymal Stromal Cell and Articular Chondrocyte Cocultures on Highly Porous Bilayer Poly-L-Lactic Acid Scaffolds Produced by Thermally Induced Phase Separation and Supplemented with Hydroxyapatite.
Ferraro W., Civilleri A., Gögele C., et al. (2024). Polymers 2024, 16,331.
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Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system.
Almeria C., Weiss R., Keck M., et al. (2024). Biotechnol Lett. 2024 Apr;46(2):279-293.
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2023

Inflammation‑inducible promoters to overexpress immune inhibitory factors by MSCs.
Selich A., Fleischauer J., Roepke T., et al. (2023). Stem Cell Res Ther. 2023 Sep 23;14(1):270.
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Dystrophin Expressing Chimeric (DEC) Cell Therapy for Duchenne Muscular Dystrophy: A First-in-Human Study with Minimum 6 Months Follow-up.
Heydemann A., Bieganski G., Wachowiak J., et al. (2023). Stem Cell Rev Rep. 2023 Jul;19(5):1340-1359.
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Bioluminescence imaging on-chip platforms for non-invasive high-content bioimaging.
Araújo-Gomes N., Zambito G., Johnbosco C., et al. (2023). Biosensors and Bioelectronics, Volume 237.
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A dialysis medium refreshment cell culture set-up for an osteoblast-osteoclast coculture.
Vis M.A.M., de Wildt B.W.M., Ito K., et al. (2023). Biotechnol Bioeng. 2023 Apr;120(4):1120-1132.
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Co-Culture of Mesenchymal Stem Cells and Ligamentocytes on Triphasic Embroidered Poly(L-lactide-co-ε-caprolactone) and Polylactic Acid Scaffolds for Anterior Cruciate Ligament Enthesis Tissue Engineering.
Gögele C., Vogt J., Hahn J., et al. (2023). International Journal of Molecular Sciences. 2023; 24(7):6714.
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Cytoprotective Effects of Human Platelet Lysate during the Xeno-Free Culture of Human Donor Corneas.
Talpan D., Salla S., Meusel L., et al. (2023). Int J Mol Sci. 2023 Feb 2;24(3):2882.
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Regenerative potential of different extracellular vesicle subpopulations derived from clonal mesenchymal stem cells in a mouse model of chemotherapy-induced premature ovarian failure.
Eslami N., Bahrehbar K., Esfandiari F., et al. (2023). Life Sci. 2023 May 15;321:121536.
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Evaluating material-driven regeneration in a tissue engineered human in vitro bone defect model.
de Wildt B.W.M., Cramer E.E.A., de Silva L.S., et al. (2023). Bone, Volume 166.
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Secretomes derived from osteogenically differentiated jaw periosteal cells inhibit phenotypic and functional maturation of CD14+ monocyte-derived dendritic cells.
Cen W., Umrath F., Salgado A.J., et al. (2023). Front. Immunol. 13:1024509.
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2022

Human placenta-derived mesenchymal stem cells transplantation in patients with acute respiratory distress syndrome (ARDS) caused by COVID-19 (phase I clinical trial): safety profile assessment.
Aghayan H.R., Salimian F., Abedini A., et al. (2022). Stem Cell Res Ther 13, 365.
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Direct addition of poly-lysine or poly-ethylenimine to the medium: A simple alternative to plate pre-coating.
Faussner A., Deininger M.M., Weber C., et al. (2022). PLoS ONE 17:7.
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Human Platelet Lysate as Alternative of Fetal Bovine Serum for Enhanced Human in vitro Bone Resorption and Remodeling.
de Wildt B.W.M., Ito K., Hofmann S. (2022). Front Immunol. 13:915277.
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A parallelized, perfused 3D triculture model of leukemia for in vitro drug testing of chemotherapeutics.
Zippel S., Dilger N., Chatterjee C., et al. (2022). Biofabrication, 14, 035011.
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Biodegradable Poly(D-L-lactide-co-glycolide) (PLGA)-Infiltrated Bioactive Glass (CAR12N) Scaffolds Maintain Mesenchymal Stem Cell Chondrogenesis for Cartilage Tissue Engineering.
Gögele C., Müller S., Belov S., et al. (2022). Cells, 11, 1577.
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Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches.
Karakaya E., Bider F., Frank A., et al. (2022). Gels, 8, 206.
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Pre-Conditioning with IFN- and Hypoxia Enhances the Angiogenic Potential of iPSC-Derived MSC Secretome.
Wang S, Umrath F, Cen W, et al. (2022). Cells ; 11, 988.
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Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells.
Nebel S., Lux M., Kuth S., et al. (2022). Bioengineering, 9, 66.
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Extracellular Matrix Synthesis and Remodeling by Mesenchymal Stromal Cells Is Context-Sensitive.
Burk J., Sassmann A., Kasper C., et al. (2022). Int. J. Mol. Sci., 23, 1758.
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2021

Phospholipid Profiles for Phenotypic Characterization of Adipose-Derived Multipotent Mesenchymal Stromal Cells.
Burk J., Melzer M., Hagen A., et al. (2022). Front. Cell Dev. Biol. 9:784405.
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Proliferation and Differentiation of Intestinal Caco-2 Cells Are Maintained in Culture with Human Platelet Lysate Instead of Fetal Calf Serum.
Wanes D, Naim HY, Dengler F. (2021). Cells; 10, 3038.
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Human platelet lysate as a replacement for fetal bovine serum in human corneal stromal keratocyte and fibroblast culture.
Seidelmann N, Duarte Campos DF, Rohde M, et al. (2021). Journal of Cellular and Molecular Medicine; 00:1–13.
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Profiling of human neural crest chemoattractant activity as replacement of fetal bovine serum for in vitro chemotaxis assays.
Dolde X, Karreman C, Wiechers M, et al. (2021). International Journal of Molecular Sciences; 22, 10079.
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Influence of Charge and Heat on the Mechanical Properties of Scaffolds from Ionic Complexation of Chitosan and Carboxymethyl Cellulose.
Dobaj Štiglic A, Kargl R, Beaumont M, et al. (2021). ACS Biomater Sci Eng.; 7(8):3618-3632.
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Placenta derived Mesenchymal Stem Cells transplantation in Type 1 diabetes: preliminary report of phase 1 clinical trial.
Madani S, Setudeh A, Aghayan HR, et al. (2021). Journal of Diabetes & Metabolic Disorders.
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Translation of Collagen Ultrastructure to Biomaterial Fabrication for Material-Independent but Highly Efficient Topographic Immunomodulation.
Ryma M, Tylek T, Liebscher J, et al. (2021). Advanced Materials.
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Platelet-enhanced plasma: Characterization of a novel candidate resuscitation fluid’s extracellular vesicle content, clotting parameters, and thrombin generation capacity.
Price J, Gardiner C, Harrison P (2021). Transfusion.
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Inducing Immunomodulatory Effects on Human Macrophages by Multifunctional NCO-sP(EO-stat-PO)/Gelatin Hydrogel Nanofibers.
Taskin M.B., Tylek T., Blum C., et al. (2021). ACS Biomater. Sci. Eng, 7, 3166−3178.
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Towards Physiologic Culture Approaches to Improve Standard Cultivation of Mesenchymal Stem Cells. (Review).
Nikolits I, Nebel S, Egger D, et al. (2021). Cells; 10:886.
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Mesenchymal stem cells’ seeded amniotic membrane as a tissue-engineered dressing for wound healing.
Aghayan HR, Hosseini MS, Gholami M, et al. (2021). Drug Deliv. and Transl. Res.
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In vitro characterisation of 3D printed platelet lysate-based bioink for potential application in skin tissue engineering.
Daikuara LY, Yue Z, Skropeta D, et al. (2021). Acta Biomaterialia 123.
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2020

Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Show Comparable Functionality to Their Autologous Origin.
Jakob M, Hambrecht M, Spiegel JL, et al. (2020). Cells; 10:33.
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Burkholderia pseudomallei triggers canonical inflammasome activation in a human primary macrophage-based infection model.
Lichtenegger S, Stiehler J, Saiger S, et al. (2020). PLoS Negl Trop Dis 14:11.
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Xeno- and transgene-free reprogramming of mesenchymal stem cells toward the cells expressing neutral markers using exosome treatments.
Valerio LSA, Sugaya K. (2020). PLoS ONE 15:10.
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Scales Isolation of Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles.
Börger V, Staubach S, Dittrich R, et al. (2020). Current Protocols in Stem Cell Biology, 55, e128. doi: 10.1002/cpsc.128.
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Regulatory-compliant conditions during cell product manufacturing enhance in vitro immunomodulatory properties of infrapatellar fat pad-derived mesenchymal stem/stromal cells.
Kouroupis D, Bowles AC, Greif DN, et al. (2020). Cytotherapy.
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Monitoring matrix remodeling in the cellular microenvironment using microrheology for complex cellular systems.
Hafner J, Grijalva D, Ludwig-Husemann A, et al. (2020). Acta Biomaterialia 111.
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Ex vivo-expanded autologous adipose tissue-derived stromal cells ensure enhanced fat graft retention in breast augmentation: A randomized controlled clinical trial.
Kølle ST, Duscher D, Taudorf M, et al. (2020). Stem Cells Transl Med.; 9(11):1277-1286.
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3D Printing of Cell Culture Devices: Assessment and Prevention of the Cytotoxicity of Photopolymers for Stereolithography.
Kreß S, Schaller-Ammann R, Feiel J, et al. (2020). Materials; 13:3011.
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CD10/Neprilysin Enrichment in Infrapatellar Fat Pad-Derived Mesenchymal Stem Cells Under Regulatory-Compliant Conditions: Implications for Efficient Synovitis and Fat Pad Fibrosis Reversal.
Kouroupis D, Bowles AC, Best T, et al. (2020). The American Journal of Sports Medicine, 1-15.
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Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells.
Karadjian M, Senger A-S, Essers C, et al. (2020). Cells; 9: 918.
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Precisely defined fiber scaffolds with 40 μm porosity induce elongation driven M2-like polarisation of human macrophages.
Tylek T, Blum C, Hrynevich A, et al. (2020). Biofabrication 12:025007.
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2019

Potential of electrospun cationic BSA fibers to guide osteogenic MSC differentiation via surface charge and fibrous topography.
Raic A, Friedrich F, Kratzer D, et al. (2019). Scientific Reports; 9:20003.
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Developing hyaluronic acid microgels for sustained delivery of platelet lysate for tissue engineering applications.
Jooybar E, Abdekhodaie MJ, Karperien M, et al. (2019). International Journal of Biological Macromolecules 144.
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Hypoxia Conditioned Mesenchymal Stem Cell-Derived Extracellular Vesicles Induce Increased Vascular Tube Formation in vitro.
Almeria C, Weiss R, Roy M, et al. (2019). Front. Bioeng. Biotechnol. 7:292
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The Power of LC-MS Based Multiomics: Exploring Adipogenic Differentiation of Human Mesenchymal Stem/Stromal Cells.
Rampler E, Egger D, Schoeny H, et al. (2019). Molecules; 24:3615.
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From 3D to 3D: isolation of mesenchymal stem/stromal cells into a three-dimensional human platelet lysate matrix.
Egger D, Oliveira A, Mallinger B, et al. (2019). Stem Cell Research & Therapy; 10:248.
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A High-Throughput Method as a Diagnostic Tool for HIV Detection in Patient-Specific Induced Pluripotent Stem Cells Generated by Different Reprogramming Methods.
Hübscher D, Rebs S, Haupt L, et al. (2019). Stem Cells International.
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Platelet lysate outperforms FCS and human serum for co-culture of primary human macrophages and hMSCs.
Tylek T, Schilling T, Schlegelmilch K, et al. (2019). Scientific Reports; 9:3533.
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Human platelet lysate current standards and future developments. Committee report.
Henschler R, Gabriel C, Schallmoser K, et al. (2019). Transfusion; 9999; 1–7.
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An injectable platelet lysate-hyaluronic acid hydrogel supports cellular activities and induces chondrogenesis of encapsulated mesenchymal stem cells.
Jooybar E, Abdekhodaie MJ, Alvi M, et al. (2019). Acta Biomaterialia; 83: 233–244.
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2018

Fetal Bovine Serum (FBS): Past – Present – Future. Consensus Report.
van der Valk J, Bieback K, Buta C, et al. (2018). ALTEX – Alternatives to animal experimentation; 35(1):99-118.
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2017

Development and Characterization of a Parallelizable Perfusion Bioreactor for 3D Cell Culture.
Egger D, Schwedhelm I, Hansmann J, et al. (2017). Bioengineering; 4, 47.
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Hypoxic Three-Dimensional Scaffold-Free Aggregate Cultivation of Mesenchymal Stem Cells in a Stirred Tank Reactor.
Egger D, Schwedhelm I, Hansmann J, et al. (2017). Bioengineering; 4, 47.
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Epigenetic Biomarker to Support Classification into Pluripotent and Non-Pluripotent Cells.
Lenz M., Goetzke R., Schenk A., Schubert C., Veeck J., Hemeda H., Koschmieder S., Zenke M., Schuppert A. & Wagner W. (2015). Scientific Reports; 5:8973.
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Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells.
Hemeda H., Wagner W. & Giebel B. (2014). Cytotherapy; 16(2):170-180.
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Epigenetic Rejuvenation of Mesenchymal Stromal Cells Derived from Induced Pluripotent Stem Cells.
Frobel F., Hemeda H., Lenz M., Abagnale G., Joussen S., Denecke B., Šarić T., Zenke M. & Wagner W. (2014). Stem Cell Reports; Vol. 3; 414–422.
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Matrix elasticity, replicative senescenece and DNA methylation patterns of mesenchymal stem cells.
Schellenberg A., Joussen S., Moser K., Hampe N., Hersche N., Hemeda H., Schnitker J., Denecke B., Qiong L., Pallua N., Zenke M., Merkel R., Hoffmann B. & Wagner W. (2014). Biomaterials; 35(24):6351-6358.
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Anticoagulant concentration is critical for cell culture with human platelet lysate.
Hemeda H., Kalz J., Walenda G., Lohmann M. & Wagner W. (2013). Cytotherapy; 15(9):1174-1181.
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Donor age of human platelet lysate affects proliferation and differentation of mesenchymal stem cells.
Lohmann M., Walenda G., Hemeda H., Joussen S., Drescher W., Jockenhoevel S., Hutschenreuter G., Zenke M. & Wagner W. (2012). PLoS ONE; 7(5): e37839.
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Human platelet lysate gel provides a novel three dimensional-matrix for enhanced culture expansion of mesenchymal stromal cells.
Walenda G., Hemeda H., Schneider R. K., Merkel R., Hoffmann B. & Wagner W. (2012). Tissue Eng Part C Methods; 18(12):924-934.
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