Publications

Proliferation and Differentiation of Intestinal Caco-2 Cells Are Maintained in Culture with Human Platelet Lysate Instead of Fetal Calf Serum.
Wanes D, Naim HY, Dengler F. (2021). Cells; 10, 3038.
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Profiling of human neural crest chemoattractant activity as replacement of fetal bovine serum for in vitro chemotaxis assays.
Dolde X, Karreman C, Wiechers M, et al. (2021). International Journal of Molecular Sciences; 22, 10079.
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Human platelet lysate as a replacement for fetal bovine serum in human corneal stromal keratocyte and fibroblast culture.
Seidelmann N, Duarte Campos DF, Rohde M, et al. (2021). Journal of Cellular and Molecular Medicine; 00:1–13.
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Influence of Charge and Heat on the Mechanical Properties of Scaffolds from Ionic Complexation of Chitosan and Carboxymethyl Cellulose.
Dobaj Štiglic A, Kargl R, Beaumont M, et al. (2021). ACS Biomater Sci Eng.; 7(8):3618-3632.
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Placenta derived Mesenchymal Stem Cells transplantation in Type 1 diabetes: preliminary report of phase 1 clinical trial.
Madani S, Setudeh A, Aghayan HR, et al. (2021). Journal of Diabetes & Metabolic Disorders.
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Translation of Collagen Ultrastructure to Biomaterial Fabrication for Material-Independent but Highly Efficient Topographic Immunomodulation.
Ryma M, Tylek T, Liebscher J, et al. (2021). Advanced Materials.
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Platelet-enhanced plasma: Characterization of a novel candidate resuscitation fluid’s extracellular vesicle content, clotting parameters, and thrombin generation capacity.
Price J, Gardiner C, Harrison P (2021). Transfusion.
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Towards Physiologic Culture Approaches to Improve Standard Cultivation of Mesenchymal Stem Cells. (Review).
Nikolits I, Nebel S, Egger D, et al. (2021). Cells; 10:886.
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In vitro characterisation of 3D printed platelet lysate-based bioink for potential application in skin tissue engineering.
Daikuara LY, Yue Z, Skropeta D, et al. (2021). Acta Biomaterialia 123.
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Mesenchymal stem cells’ seeded amniotic membrane as a tissue-engineered dressing for wound healing.
Aghayan HR, Hosseini MS, Gholami M, et al. (2021). Drug Deliv. and Transl. Res.
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2020

Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Show Comparable Functionality to Their Autologous Origin.
Jakob M, Hambrecht M, Spiegel JL, et al. (2020). Cells; 10:33.
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Burkholderia pseudomallei triggers canonical inflammasome activation in a human primary macrophage-based infection model.
Lichtenegger S, Stiehler J, Saiger S, et al. (2020). PLoS Negl Trop Dis 14:11.
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Xeno- and transgene-free reprogramming of mesenchymal stem cells toward the cells expressing neutral markers using exosome treatments.
Valerio LSA, Sugaya K. (2020). PLoS ONE 15:10.
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Regulatory-compliant conditions during cell product manufacturing enhance in vitro immunomodulatory properties of infrapatellar fat pad-derived mesenchymal stem/stromal cells.
Kouroupis D, Bowles AC, Greif DN, et al. (2020). Cytotherapy.
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Monitoring matrix remodeling in the cellular microenvironment using microrheology for complex cellular systems.
Hafner J, Grijalva D, Ludwig-Husemann A, et al. (2020). Acta Biomaterialia 111.
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Ex vivo-expanded autologous adipose tissue-derived stromal cells ensure enhanced fat graft retention in breast augmentation: A randomized controlled clinical trial.
Kølle ST, Duscher D, Taudorf M, et al. (2020). Stem Cells Transl Med.; 9(11):1277-1286.
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3D Printing of Cell Culture Devices: Assessment and Prevention of the Cytotoxicity of Photopolymers for Stereolithography.
Kreß S, Schaller-Ammann R, Feiel J, et al. (2020). Materials; 13:3011.
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CD10/Neprilysin Enrichment in Infrapatellar Fat Pad-Derived Mesenchymal Stem Cells Under Regulatory-Compliant Conditions: Implications for Efficient Synovitis and Fat Pad Fibrosis Reversal.
Kouroupis D, Bowles AC, Best T, et al. (2020). The American Journal of Sports Medicine, 1-15.
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Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells.
Karadjian M, Senger A-S, Essers C, et al. (2020). Cells; 9: 918.
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Precisely defined fiber scaffolds with 40 μm porosity induce elongation driven M2-like polarisation of human macrophages.
Tylek T, Blum C, Hrynevich A, et al. (2020). Biofabrication 12:025007.
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2019

Potential of electrospun cationic BSA fibers to guide osteogenic MSC differentiation via surface charge and fibrous topography.
Raic A, Friedrich F, Kratzer D, et al. (2019). Scientific Reports; 9:20003.
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Developing hyaluronic acid microgels for sustained delivery of platelet lysate for tissue engineering applications.
Jooybar E, Abdekhodaie MJ, Karperien M, et al. (2019). International Journal of Biological Macromolecules 144.
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Hypoxia Conditioned Mesenchymal Stem Cell-Derived Extracellular Vesicles Induce Increased Vascular Tube Formation in vitro.
Almeria C, Weiss R, Roy M, et al. (2019). Front. Bioeng. Biotechnol. 7:292
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The Power of LC-MS Based Multiomics: Exploring Adipogenic Differentiation of Human Mesenchymal Stem/Stromal Cells.
Rampler E, Egger D, Schoeny H, et al. (2019). Molecules; 24:3615.
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From 3D to 3D: isolation of mesenchymal stem/stromal cells into a three-dimensional human platelet lysate matrix.
Egger D, Oliveira A, Mallinger B, et al. (2019). Stem Cell Research & Therapy; 10:248.
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A High-Throughput Method as a Diagnostic Tool for HIV Detection in Patient-Specific Induced Pluripotent Stem Cells Generated by Different Reprogramming Methods.
Hübscher D, Rebs S, Haupt L, et al. (2019). Stem Cells International.
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Platelet lysate outperforms FCS and human serum for co-culture of primary human macrophages and hMSCs.
Tylek T, Schilling T, Schlegelmilch K, et al. (2019). Scientific Reports; 9:3533.
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Human platelet lysate current standards and future developments. Committee report.
Henschler R, Gabriel C, Schallmoser K, et al. (2019). Transfusion; 9999; 1–7.
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An injectable platelet lysate-hyaluronic acid hydrogel supports cellular activities and induces chondrogenesis of encapsulated mesenchymal stem cells.
Jooybar E, Abdekhodaie MJ, Alvi M, et al. (2019). Acta Biomaterialia; 83: 233–244.
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2018

Fetal Bovine Serum (FBS): Past – Present – Future. Consensus Report.
van der Valk J, Bieback K, Buta C, et al. (2018). ALTEX – Alternatives to animal experimentation; 35(1):99-118.
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2017

Development and Characterization of a Parallelizable Perfusion Bioreactor for 3D Cell Culture.
Egger D, Schwedhelm I, Hansmann J, et al. (2017). Bioengineering; 4, 47.
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Hypoxic Three-Dimensional Scaffold-Free Aggregate Cultivation of Mesenchymal Stem Cells in a Stirred Tank Reactor.
Egger D, Schwedhelm I, Hansmann J, et al. (2017). Bioengineering; 4, 47.
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Epigenetic Biomarker to Support Classification into Pluripotent and Non-Pluripotent Cells.
Lenz M., Goetzke R., Schenk A., Schubert C., Veeck J., Hemeda H., Koschmieder S., Zenke M., Schuppert A. & Wagner W. (2015). Scientific Reports; 5:8973.
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Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells.
Hemeda H., Wagner W. & Giebel B. (2014). Cytotherapy; 16(2):170-180.
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Epigenetic Rejuvenation of Mesenchymal Stromal Cells Derived from Induced Pluripotent Stem Cells.
Frobel F., Hemeda H., Lenz M., Abagnale G., Joussen S., Denecke B., Šarić T., Zenke M. & Wagner W. (2014). Stem Cell Reports; Vol. 3; 414–422.
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Matrix elasticity, replicative senescenece and DNA methylation patterns of mesenchymal stem cells.
Schellenberg A., Joussen S., Moser K., Hampe N., Hersche N., Hemeda H., Schnitker J., Denecke B., Qiong L., Pallua N., Zenke M., Merkel R., Hoffmann B. & Wagner W. (2014). Biomaterials; 35(24):6351-6358.
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Anticoagulant concentration is critical for cell culture with human platelet lysate.
Hemeda H., Kalz J., Walenda G., Lohmann M. & Wagner W. (2013). Cytotherapy; 15(9):1174-1181.
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Donor age of human platelet lysate affects proliferation and differentation of mesenchymal stem cells.
Lohmann M., Walenda G., Hemeda H., Joussen S., Drescher W., Jockenhoevel S., Hutschenreuter G., Zenke M. & Wagner W. (2012). PLoS ONE; 7(5): e37839.
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Human platelet lysate gel provides a novel three dimensional-matrix for enhanced culture expansion of mesenchymal stromal cells.
Walenda G., Hemeda H., Schneider R. K., Merkel R., Hoffmann B. & Wagner W. (2012). Tissue Eng Part C Methods; 18(12):924-934.
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