ELAREM™ Technology

HUMAN PLATELET LYSATE TECHNOLOGY

ELAREM™ – A SUPERIOR TECHNOLOGY TO PRODUCE SAFE & EFFICIENT CELL CULTURE SUPPLEMENTS

As a company focusing on human Platelet Lysate (hPL) development and supply for many years, we have a rich knowledge base. On this technology page, we want to share our knowledge with you. Take an inside look into the technology behind the name ELAREM™.

WHAT IS THE ELAREM™ PLATFORM?

Our ELAREM™ platform is a portfolio of cell culture media based on human platelets. Due to their high growth factor content, human platelets are rich in nutrients necessary for in vitro cell expansion. The solution that extracts these relevant factors out of human platelets is called human Platelet Lysate.

All our ELAREM™ products are xeno-free cell culture supplements. This means that the cell culture supplements and potential related products do not contain any animal components.

ELAREM™ – ONE SOLUTION AT A TIME

With our human Platelet Lysate product range ELAREM™, we created cell culture supplements that fit the needs of every application field.

We strive to deliver tailored cell culture supplements covering all needs of cell expansion in academic research, pre-clinical research and cellular therapy.

ELAREM™ PRIME

ELAREM™ Prime is the worldwide first affordable cell culture supplement based on human platelets offering the same benefits as FBS – in an animal serum-free environment.

 

ELAREM™ PERFORM

ELAREM™ Perform is a human Platelet Lysate that meets the needs of research and development. The safe and performance-increasing cell growth promoter ensures efficient lab processes and a xeno-free cell culture environment.

ELAREM™ Perform-FD is additionally fibrinogen-depleted and does not require anticoagulant addition.

ELAREM™ ULTIMATE

ELAREM™ Ultimate-FDi is a virus-inactivated and fibrinogen-depleted human Platelet Lysate suited to clinical trial and therapeutic development needs. ELAREM™ Ultimate-FDi contains abundant growth factors and cytokines necessary for industrial in vitro cell expansion.

ELAREM™ MATRIX

ELAREM Matrix

ELAREM™ Matrix Kit provides a patent-protected gel scaffold based on human Platelet Lysate. The system is suitable for 2D and 3D cell culture applications. The animal component-free technology combines both a nutrient source and a viscous scaffold in one – resembling the natural cell environment.

PERFORMANCE OF HUMAN PLATELET LYSATE

Human Platelet Lysate contains abundant growth factors and cytokines derived from human platelets. Those nutrients have been shown to stimulate cellular proliferation and maintain phenotype and differentiation potential of various cells.

ELAREM™ SUPPORTS THE IN VITRO EXPANSION OF VARIOUS PRIMARY CELLS AND CELL LINES

Our human Platelet Lysates can be used for a numerous amount of cell types.

To achieve high cell proliferation rates, we recommend adding 10% (v/v) ELAREM™ human Platelet Lysate to the complete cell culture medium.

Final concentration of ELAREM™ human Platelet Lysate in cell culture medium can vary depending on cell type and experimental conditions. It is recommended to determine the optimal concentration – between 1% and 10% (v/v) – for the cells of interest.

A small extract of cells that profit from the nutrient-rich in vitro environment provided by human Platelet Lysate:

  • Mouse Myoblastoma Cells
  • Human continuous tumor cells
  • Keratinocyte cell line from adult human skin (HaCaT)
  • Human Embryonic Kidney 293 cells
  • Cervical cancer cells (HeLa)
  • Dermal fibroblasts
  • Human leukemia cell lines
  • Human umbilical vein endothelial cells
  • Mesenchymal Stem Cells
  • Dermal keratinocytes
  • Vero cells

Based on a various number of publications and customer feedbacks, we have gathered data on a huge amount of cell types cultivated with human Platelet Lysate. Please contact us if you want to know specific data.

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TIME & MONEY SAVING

The high concentration of growth factors and proteins accelerates cell proliferation, leading to faster results with less medium supplementation.

SAFETY

In addition to being free from ethical concerns, human Platelet Lysate allows for much better traceability and does not pose a risk of animal-derived pathogen and prion transmission.

REPRODUCIBILITY

Our ELAREM™ human Platelet Lysate ensures a high lot-to-lot consistency. This eliminates the need of FBS-related batch testing and ensures reproducible results – while human Platelet Lysate is suitable for human or animal primary cells and cell lines.

WHAT IS SPECIAL ABOUT OUR NEW PRODUCT ELAREM™ PRIME?

ELAREM Prime 50ml human Platelet Lysate PL BioScience

WHAT IS ELAREM™ PRIME?

ELAREM™ Prime is a worldwide unique cell culture solution covering future-oriented needs. The affordable cell growth promoter based on human Platelet Lysate enables a simple switch from animal serum-supplemented to xeno-free cell culture conditions. ELAREM™ Prime offers the same benefits as FBS, but in an animal component-free environment.

The manufacture of ELAREM™ Prime is based on the recycling of expired human platelet donations, so we do not depend on the slaughter industry. Therefore we offer a price-stable and affordable cell culture medium comparable to FBS.

3 GOOD REASONS TO USE ELAREM™ PRIME INSTEAD OF FBS

ELAREM™ Prime enables a simple switch to animal-free conditions and offers multiple benefits.

ELAREM™ Prime provides consistent trial results without the need of batch testing.

The cellular growth performance is comparable to FBS-supplemented cell cultures.

Human Platelet Lysate is free from ethical concerns and provided by a sustainable production source.

Cell Growth performance hASC of ELAREM Prime vs. FBS

HOW IS HUMAN PLATELET LYSATE PRODUCED?

Human Platelet Lysate is derived from transfusion-approved human platelets (thrombocytes). Due to safety reasons, the storage capability of platelets required for blood transfusion is very limited. The platelets that cannot be used for blood transfusions any more are reprocessed to our cell culture medium. This represents a sustainable source that allows, unlike FBS, for independency from the slaughter industry.

RAW MATERIAL

Human platelet units are supplied by licensed blood donation centres where donors have been tested.

TRANSPORT

Platelet units need to be stored frozen within the entire transportation prior to manufacturing.

MANUFACTURING

Platelet units are subjected to a standardized manufacturing process. Raw materials, equipment and facilities are in compliance with applicable cGMP regulations.

FINAL PRODUCT

The final product is tested according to standardized validation tests while all criteria are documented.

CUSTOMER

The customers benefit from our look-back procedure enabling the tracing of every production step.

MANUFACTURING OF HUMAN PLATELET LYSATE

Quality and security is important when producing human Platelet Lysate. Therefore we produce our ELAREM™ Platform in qualified Controlled Environment Rooms (CERs) and cGMP compliant clean room areas.

PRODUCT REFERENCES

You find more references and publications on our Publications site.

Regulatory-compliant conditions during cell product manufacturing enhance in vitro immunomodulatory properties of infrapatellar fat pad-derived mesenchymal stem/stromal cells.
Kouroupis D, Bowles A, Greif D, et al. (2020). Cytotherapy.
View article

CD10/Neprilysin Enrichment in Infrapatellar Fat Pad-Derived Mesenchymal Stem Cells Under Regulatory-Compliant Conditions: Implications for Efficient Synovitis and Fat Pad Fibrosis Reversal.
Kouroupis D, Bowles A, Best T, et al. (2020). The American Journal of Sports Medicine, 1-15.
View article

Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells.
Karadjian M, Senger A-S, Essers C, et al. (2020). Cells; 9: 918.
View article

(1) Read the publication: https://www.mdpi.com/2073-4409/9/4/918

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Please contact us:

support@pl-bioscience.com

+49 (0) 241 95719-100