As a company focusing on human Platelet Lysate (hPL) development and supply for many years, we have a rich knowledge base. On this technology page, we want to share our knowledge with you. Take an inside look into the technology behind the name ELAREM™
Our ELAREM™ platform is a portfolio of cell culture media based on human platelets. Due to their high growth factor content, human platelets are rich in nutrients necessary for in vitro cell expansion. The solution that extracts these relevant factors out of human platelets is called human Platelet Lysate.
All our ELAREM™ products are xeno-free cell culture supplements. This means that the cell culture supplements and potential related products do not contain any animal components.
With our human Platelet Lysate product range ELAREM™, we created cell culture supplements that fit the needs of every application field.
We strive to deliver tailored cell culture supplements covering all needs of cell expansion in academic research, pre-clinical research and cellular therapy.
ELAREM™ Prime is the worldwide first affordable cell culture supplement based on human platelets offering the same benefits as FBS – in an animal serum-free environment.
ELAREM™ Perform is a human Platelet Lysate that meets the needs of research and development. The safe and performance-increasing cell growth promoter ensures efficient lab processes and a xeno-free cell culture environment.
ELAREM™ Perform-FD is additionally fibrinogen-depleted and does not require anticoagulant addition.
ELAREM™ Ultimate-FDi is a virus-inactivated and fibrinogen-depleted human Platelet Lysate suited to clinical trial and therapeutic development needs. ELAREM™ Ultimate-FDi contains abundant growth factors and cytokines necessary for industrial in vitro cell expansion.
ELAREM™ Matrix Kit provides a patent-protected gel scaffold based on human Platelet Lysate. The system is suitable for 2D and 3D cell culture applications. The animal component-free technology combines both a nutrient source and a viscous scaffold in one – resembling the natural cell environment.
Human Platelet Lysate contains abundant growth factors and cytokines derived from human platelets. Those nutrients have been shown to stimulate cellular proliferation and maintain phenotype and differentiation potential of various cells.
Our human Platelet Lysates can be used for a numerous amount of cell types.
To achieve high cell proliferation rates, we recommend adding 10% (v/v) ELAREM™ human Platelet Lysate to the complete cell culture medium.
Final concentration of ELAREM™ human Platelet Lysate in cell culture medium can vary depending on cell type and experimental conditions. It is recommended to determine the optimal concentration – between 1% and 10% (v/v) – for the cells of interest.
A small extract of cells that profit from the nutrient-rich in vitro environment provided by human Platelet Lysate:
Based on a various number of publications and customer feedbacks, we have gathered data on a huge amount of cell types cultivated with human Platelet Lysate. Please contact us if you want to know specific data.
The high concentration of growth factors and proteins accelerates cell proliferation, leading to faster results with less medium supplementation.
In addition to being free from ethical concerns, human Platelet Lysate allows for much better traceability and does not pose a risk of animal-derived pathogen and prion transmission.
Our ELAREM™ human Platelet Lysate ensures a high lot-to-lot consistency. This eliminates the need of FBS-related batch testing and ensures reproducible results – while human Platelet Lysate is suitable for human or animal primary cells and cell lines.
ELAREM™ Prime is a worldwide unique cell culture solution covering future-oriented needs. The affordable cell growth promoter based on human Platelet Lysate enables a simple switch from animal serum-supplemented to xeno-free cell culture conditions. ELAREM™ Prime offers the same benefits as FBS, but in an animal component-free environment.
The manufacture of ELAREM™ Prime is based on the recycling of expired human platelet donations, so we do not depend on the slaughter industry. Therefore we offer a price-stable and affordable cell culture medium comparable to FBS.
ELAREM™ Prime provides consistent trial results without the need of batch testing.
The cellular growth performance is comparable to FBS-supplemented cell cultures.
Human Platelet Lysate is free from ethical concerns and provided by a sustainable production source.
Human Platelet Lysate is derived from transfusion-approved human platelets (thrombocytes). Due to safety reasons, the storage capability of platelets required for blood transfusion is very limited. The platelets that cannot be used for blood transfusions any more are reprocessed to our cell culture medium. This represents a sustainable source that allows, unlike FBS, for independency from the slaughter industry.
Human platelet units are supplied by licensed blood donation centres where donors have been tested.
Platelet units need to be stored frozen within the entire transportation prior to manufacturing.
Platelet units are subjected to a standardized manufacturing process. Raw materials, equipment and facilities are in compliance with applicable cGMP regulations.
The final product is tested according to standardized validation tests while all criteria are documented.
The customers benefit from our look-back procedure enabling the tracing of every production step.
Quality and security is important when producing human Platelet Lysate. Therefore we produce our ELAREM™ Platform in qualified Controlled Environment Rooms (CERs) and cGMP compliant clean room areas.
Regulatory-compliant conditions during cell product manufacturing enhance in vitro immunomodulatory properties of infrapatellar fat pad-derived mesenchymal stem/stromal cells.
Kouroupis D, Bowles A, Greif D, et al. (2020). Cytotherapy.
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CD10/Neprilysin Enrichment in Infrapatellar Fat Pad-Derived Mesenchymal Stem Cells Under Regulatory-Compliant Conditions: Implications for Efficient Synovitis and Fat Pad Fibrosis Reversal.
Kouroupis D, Bowles A, Best T, et al. (2020). The American Journal of Sports Medicine, 1-15.
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Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells.
Karadjian M, Senger A-S, Essers C, et al. (2020). Cells; 9: 918.
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